Reporter
Strong+CFP

Part:BBa_K320005

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)

Constitutive expression of CFP

This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(BBa_K320002) and a CFP biobrick (BBa_E0020).

This BioBrick was built to test the constitutive promoter (BBa_K320002).

It has only been characterized in E. coli (see the Experience). We are currently running experiments to characterize it in Asaia to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia new chassis].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
    Illegal NheI site found at 146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/constitutive
Parameters
None